Induction of apomixis by dimethyl sulfoxide (DMSO) and genetic identification of apomictic plants in cassava

文献类型： 外文期刊

第一作者： Chen, Xia

作者： Chen, Xia;Lai, Hang-Gui;Sun, Qi;Liu, Jin-Ping;Chen, Song-Bi;Zhu, Wen-Li

作者机构：

关键词： Manihot esculenta; induced apomixis; DMSO; EST-SSR markers; SRAP markers

期刊名称：BREEDING SCIENCE （ 影响因子：2.086； 五年影响因子：2.632 ）

ISSN： 1344-7610

年卷期： 2018 年 68 卷 2 期

页码：

收录情况： SCI

摘要： Apomixis, or asexual seed formation, is of great value for plant breeding and seed production, and is desirable in modern agriculture, but natural apomixis occurs in cassava at very low frequency. In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/v) dimethyl sulfoxide (DMSO) and the results showed that 1.5% DMSO treatment was most effective for the induction of apomictic seed formation in cassava cultivar SC5 with the highest percentages of fruit set and true apomictic seeds. The germinated seedlings resembled their parents and displayed no morphological characteristics of cassava polyploid. Flow cytometry and chromosome counting showed that these plants were uniform diploids. Analysis of 34 DMSO-induced cassava progenies by the expressed sequence tag-simple sequence repeat (EST-SSR) and sequence-related amplified polymorphism (SRAP) markers showed that three true apomictic seeds were obtained from the group of SC5 treated with 1.5% DMSO.

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