Genetic characterization of extraintestinal Escherichia coli isolates from chicken, cow and swine

文献类型： 外文期刊

第一作者： Chen, Li

作者： Chen, Li;Yassin, Afrah Kamal;Zhang, Jilei;Zhang, Yuanyuan;Yang, Yi;Wang, Leyi;Wang, Leyi;Yassin, Afrah Kamal;Gong, Jiansen;Qi, Kezong;Ganta, Roman R.;Han, Xiangan;Wang, Chengming

作者机构：

关键词： Escherichia coli; Antimicrobial resistance; Whole-genome sequencing; MLST sequence type; VFDB; Phylogenetic analysis

期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）

ISSN： 2191-0855

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： Phenotypic determination of antimicrobial resistance in bacteria is very important for diagnosis and treatment, but sometimes this procedure needs further genetic evaluation. Whole-genome sequencing plays a critical role in deciphering and advancing our understanding of bacterial evolution, transmission, and surveillance of antimicrobial resistance. In this study, whole-genome sequencing was performed on nineteen clinically extraintestinal Escherichia coli isolates from chicken, cows and swine and showing different antimicrobial susceptibility. A total of 44 different genes conferring resistance to 11 classes of antimicrobials were detected in 15 of 19 E. coli isolates (78.9%), and 22 types of plasmids were detected in 15/19 (78.9%) isolates. In addition, whole-genome sequencing of these 19 isolates identified 111 potential virulence factors, and 53 of these VFDB-annotated genes were carried by all these 19 isolates. Twelve different virulence genes were identified while the most frequent ones were gad (glutamate decarboxylase), iss (increased serum survival) and lpfA (long polar fimbriae). All isolates harbored at least one of the virulence genes. The findings from comparative genomic analyses of the 19 diverse E. coli isolates in this study provided insights into molecular basis of the rising multi-drug resistance in E. coli.

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