Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice

文献类型： 外文期刊

第一作者： Shao, Jingxia

作者： Shao, Jingxia;Shao, Jingxia;Haider, Imran;Hussain, Rana Muhammad Fraz;Meijer, Annemarie H.;Wang, Mei;Ouwerkerk, Pieter B. F.;Haider, Imran;Bouwmeester, Harro J.;Xiong, Lizhong;Zhu, Xiaoyi;Overnas, Elin;Zhang, Gaisheng;Wang, Mei;Haider, Imran;Bouwmeester, Harro J.

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2018 年 13 卷 7 期

页码：

收录情况： SCI

摘要： The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序