Immortalization of chicken embryonic liver-derived cell line by stable expression of hMRP18S-2 for serotype 4 fowl adenovirus propagation

文献类型： 外文期刊

第一作者： Feng, Lei

作者： Feng, Lei;Chen, Li;Yun, Junwen;Bi, Zhixiang;Tang, Yinghua;Wu, Peipei;Hou, Jibo;Feng, Lei;Chen, Li;Yun, Junwen;Bi, Zhixiang;Tang, Yinghua;Wu, Peipei;Hou, Jibo;Feng, Lei;Chen, Li;Bi, Zhixiang;Tang, Yinghua;Wu, Peipei;Hou, Jibo

作者机构：

关键词： Chicken embryo liver cells (CEL cells); Fowl adenovirus (FAdV); hMRP18S-2; Immortalization; Cell cycle related genes

期刊名称：BIOLOGICALS （ 影响因子：1.856； 五年影响因子：2.05 ）

ISSN： 1045-1056

年卷期： 2018 年 54 卷

页码：

收录情况： SCI

摘要： Inclusion body hepatitis and hydropericardium-hepatitis syndrome caused by serotype 4 fowl adenovirus (FAdV-4) have emerged in China since 2013. FAdV is usually propagated in primary chicken embryonic liver cells or embryo yolk sac. The aim of this work was to develop an immortalized CEL cell line by stable expression of human mitochondrial ribosomal protein 18S-2, named CEL-hMRP18S-2 cells, for the propagation of FAdV-4. The maximum cell density of CEL-hMRP18S-2 cells could reach 2.65 x 10(6) cells/ml in four-days culture. According to the mRNA levels of cell-cycle related genes in CEL-hMRP18S-2 cells tested by qRT-PCR, we speculated that the transformation of hMRP18S-2 into CEL cells caused the functional inactivation of p53 and the significant down regulation of p15(INK4b) might cause the hyperphosphorylated form of Rb, releasing E2F-1 factor and enhancing the E2F-dependent transcription for cell cycle progression. It was suspected that the up-regulated c-Myc mRNA level at the initial period of immortalization might prompt transformed cells through the G0-G1 checkpoint. The normal CPE was observed in CEL-hMRP18S-2 cells infected by FAdV-4 and microcarrier suspension culture performed for FAdV-4 propagation with 9.0 lgTCID(50)/ml suggested that CEL-hMRP18S-2 cells could be a useful continuous cell line for isolation of wild FAdV and production of FAdV-inactivated vaccine.

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