Characterization of a Novel Semi-dwarf GID1 Allele Identifies an Amino Acid Required for Its Interaction with SLR1 in Rice

文献类型： 外文期刊

第一作者： Qin, Yanling

作者： Qin, Yanling;Chun, Yan;Zhao, Jinfeng;Fang, Jingjing;Zafar, Syed Adeel;Li, Xueyong;Wang, Minxia;Qin, Yanling;Guo, Baotai

作者机构：

关键词： Rice; Gibberellins; GID1; SLR1; Dwarf

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN： 0721-7595

年卷期： 2018 年 37 卷 3 期

页码：

收录情况： SCI

摘要： Gibberellins (GAs) are a group of endogenous phytohormones, playing important roles in plant growth and development. GA-induced interaction between the receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1) and the DELLA repressor protein SLENDER RICE1 (SLR1) is critical to release GA signaling. In this study, a semi-dwarf mutant s1-1 was isolated from the rice variety Nipponbare (Oryza sativa, japonica) by EMS treatment, which exhibited significantly reduced internodes determining a dn-type dwarf pattern. GA-response experiments showed that the target gene in s1-1 is involved in GA signaling pathway. By mapping and sequencing, a novel GID1 allele with a single amino acid substitution from proline to leucine at the 158th position was identified in s1-1. GID1(P158L) could only interact with SLR1 at a high concentration of GA(3) (10(-5) M), but not at a low concentration (0 and 10(-6) M). GID1(P158L) may reduce the degree of the interaction between GID1 and SLR1 at a physiological concentration of GA(3), thereby contributing to the dwarf phenotype of s1-1. Our studies identified an important residue of GID1 required for GID1 and SLR1 interaction and provided a new semi-dwarf rice genetic resource.

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