Efficacy of Cry1Ac protein against gypsy moth and fall webworm in transgenic poplar (Populus davidiana x Populus bolleana) by bioassay

文献类型： 外文期刊

第一作者： Ding, Liping

作者： Ding, Liping;Chen, Yajuan;Wang, Hongzhi;Wei, Jianhua

作者机构：

关键词： Cry1Ac; poplar; transgenic plant; insect bioassay

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2018 年 98 卷 4 期

页码：

收录情况： SCI

摘要： A modified Bacillus thuringiensis (Bt) crylAc gene was introduced into poplar 'Shanxin' (Populus davidiana x Populus bolleana) by Agrobacterium-mediated transformation. Seventeen cry1AAc transgenic lines were regenerated. The integration and expression of the cr1lAc gene in these transgenic lines were analyzed by polymerase chain reaction (PCR) and reverse-transcription quantitative PCR, respectively. Variable amounts of cr1lAc mRNA accumulated in different transgenic plants. The expression of Cr1lAc toxin protein in transgenic plants was analyzed by enzyme-linked immunosorbent assay, the concentration ranged from 0.12 to 21.00 mu pg (g-)1. The concentration of Cr1lAc protein was highly consistent with mRNA amount in each transgenic line. Leaf section bioassays were conducted using the larvae of both gypsy moth (Lymantria dispar) and fall webworm (Hyphantria cunea). Those plants, except lines 10 and 11, caused rapid mortality of all gypsy moth and fall webworm larvae with almost no defoliation, while lines 10 and 11, with Cr1lAc protein concentrations of 0.12 and 0.76 mu pg (g-)1, respectively, provided relatively weak insect protection. Results of insect bioassays indicate that Cr1lAc protein expression over 1.3 pg (g-1) consistently produced high insect mortality for both species of moths. The correlation between concentration of Cr1lAc protein and insect mortality is important to understand for managing insect pests with transgenic plants. Transgenic poplars with high insect resistance will be useful tools for managing Lepidoptera pests of these trees.

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