Changes in apoptosis factors and activation of caspase-3 in tilapia muscle during storage

文献类型： 外文期刊

第一作者： He, Yan-fu

作者： He, Yan-fu;Huang, Hui;Li, Lai-hao;Yang, Xian-qing;Hao, Shu-xian;Zhao, Yong-qiang;He, Yan-fu

作者机构：

关键词： Tilapia muscle; ATP content; caspase-3 activity; cytochrome c; Bcl-2; Bax

期刊名称：INTERNATIONAL JOURNAL OF FOOD PROPERTIES （ 影响因子：2.727； 五年影响因子：2.938 ）

ISSN： 1094-2912

年卷期： 2018 年 21 卷 1 期

页码：

收录情况： SCI

摘要： The activation of the apoptosis pathway in tilapia muscle during postmortem storage was studied. Changes in caspase-3 activity, ATP content, cytochrome c levels, and ratio of Bcl-2/Bax levels of tilapia muscle were observed during postmortem storage at 20 degrees C. Caspase-3 activity was found to be significantly increased at first, followed by a decrease (P<0.05); the highest caspase-3 activity was observed at 1h. The ATP content decreased significantly (P<0.05), and almost exhausted after 10h storage. The cytochrome c level in the cytosol showed a significant increase after 5h of storage (P<0.05), while the mitochondrial cytochrome c levels showed a decrease. The Bcl-2/Bax ratio was stable from 0-5h, followed by a rapid decreased at 10-20h and a significant increased after 20h (P<0.05), suggesting that the apoptosis process occurred until 20h of postmortem storage. Thus, we concluded that the availability of ATP and the increase in cytosolic cytochrome c levels are essential for the activation of caspase-3, and that the former partly limits caspase-3 activity.

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