Live Attenuated Pru:Delta cdpk2 Strain of Toxoplasma gondii Protects Against Acute, Chronic, and Congenital Toxoplasmosis

文献类型： 外文期刊

第一作者： Wang, Jin-Lei

作者： Wang, Jin-Lei;Li, Ting-Ting;Chen, Kai;Yang, Wen-Bin;Bai, Meng-Jie;Huang, Si-Yang;Zhu, Xing-Quan;Elsheikha, Hany M.;Cong, Wei;Yang, Wen-Bin;Huang, Si-Yang;Zhu, Xing-Quan

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关键词： Toxoplasma gondii; vaccination; Ca2+-dependent protein kinase 2; attenuated vaccine; protective immunity

期刊名称：JOURNAL OF INFECTIOUS DISEASES （ 影响因子：5.226； 五年影响因子：5.802 ）

ISSN： 0022-1899

年卷期： 2018 年 218 卷 5 期

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收录情况： SCI

摘要： Background. The threat of Toxoplasma gondii infection in immunocompromised individuals and pregnant women necessitates the development of a safe and effective vaccine. Here, we examined the immune protection conferred by a live attenuated strain of T. gondii. Methods. We tested the efficacy of intraperitoneal vaccination using 500 Ca2+-dependent protein kinase 2 (cdpk2)-deficient tachyzoites of T. gondii Pru strain against acute, chronic, and congenital toxoplasmosis in mice. The kinetics of antibody response, cytokines, and other quantifiable correlates of protection against T. gondii infection were determined. Results. Vaccination with Pru:Delta cdpk2 induced a high level of anti-T. gondii immunoglobulin G titer, type 1 T-helper (Th1) response at 28 days postvaccination, and a mixed Th1/type 2 T-helper response at 70 days postvaccination. All vaccinated mice survived a heterologous challenge with 1000 tachyzoites of RH or ToxoDB#9 (PYS or TgC7) strains. Also, vaccination protected against homologous infection with 20 T. gondii Pru cysts, and improved pregnancy outcome by reducing parasite cyst load in the brain, maintaining litter size and body weight of pups born to vaccinated dams challenged with 10 Pru cysts compared to pups born to unvaccinated dams. Conclusions. The use of T. gondii Pru:Delta cdpk2 mutant strain represents a promising approach to protection against acute, chronic, and congenital toxoplasmosis in mice.

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