Effects of applying Lactobacillus plantarum and Chinese gallnut tannin on the dynamics of protein degradation and proteases activity in alfalfa silage

文献类型： 外文期刊

第一作者： Li, X.

作者： Li, X.;Hou, J.;Wu, Z.;Yu, Z.;Tian, J.;Zhang, Q.;Jiang, Y.

作者机构：

关键词： alfalfa silage; Lactobacillus plantarum; protease; proteolysis; tannin

期刊名称：GRASS AND FORAGE SCIENCE （ 影响因子：2.63； 五年影响因子：2.52 ）

ISSN： 0142-5242

年卷期： 2018 年 73 卷 3 期

页码：

收录情况： SCI

摘要： The effects of Lactobacillus plantarum (LP) and Chinese gallnut (Rhus chinensis Mill) tannin on the fermentation quality, nitrogen distribution, protein fractions and proteases activity of alfalfa (Medicago sativa) silage were studied. Additives added to alfalfa forage (approximately 40% DM) were LP (1x10(6)cfu/g FW) plus sucrose (4g/kg FW) (LP+S), LP (1x10(6)cfu/g FW) plus commercial cellulase (0.1g/kg FW) (LP+C) and Chinese gallnut tannin at two levels (20 and 50g/kg DM) (TA 2% and TA 5%). The control was sprayed with the same volume of distilled water. Silage was sampled and analysed on days 1, 3, 5, 7, 14, 21, 28 and 35. The results showed that the degradation of protein to nonprotein nitrogen took place mainly during the first 3days, while the degradation of peptides and free amino acids occurred throughout the ensiling process. All additives lowered nonprotein nitrogen and free amino acids nitrogen proportion during the ensiling. Additive TA 5% was the most effective to inhibit proteolysis among the four additives, followed by LP+S. They inhibited the activities of all three plant proteases and decreased production of nonprotein nitrogen, free amino acids and ammonia nitrogen during the ensiling process.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序