Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus

文献类型： 外文期刊

第一作者： Sun, Yan-gang

作者： Sun, Yan-gang;Zhang, Gaiping;Sun, Yan-gang;Li, Rui;Qiao, Songlin;Zhi, Yubao;Chen, Xin-xin;Li, Xuewu;Deng, Ruiguang;Zhang, Gaiping;Jiang, Longguang;Xie, Sha;Zhang, Gaiping;Wu, Jiawei;Zhang, Gaiping;Zhang, Gaiping

作者机构：

关键词： Interaction; Porcine epidemic diarrhea virus; Porcine aminopeptidase N

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2018 年 117 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea (PED) has caused huge economic losses to the global pork industry. Infection by its causative agent PED virus (PEDV), an Alpha-coronavirus, was previously proven to be mediated by its spike (S) glycoprotein and a cellular receptor porcine aminopeptidase N (pAPN). Interestingly, some recent studies have indicated that pAPN is not a functional receptor for PEDV. To date, there is a lack of a direct evidence for the interaction between pAPN and PEDV S protein in vitro. Here, we prepared pAPN ectodomain and the truncated variants of PEDV S protein in Drosophila S2 cells. These recombinant proteins were homogeneous after purification by metal-affinity and size-exclusion chromatography. We then assayed the purified target proteins through immunogenicity tests, PEDV binding interference assays, circular dichroism (CD) measurements, pAPN activity assay and structural determination, demonstrating that they were biologically functional. Finally, we characterized their interactions by gel filtration chromatography, native-polyacrylamide gel electrophoresis (PAGE) and surface plasmon resonance (SPR) analyses. The results showed that their affinities were too low to form complexes, which suggest that pAPN may be controversial as the genuine receptor for PEDV. Therefore, further research needs to be carried out to elucidate the interaction between PEDV and its genuine receptor. (C) 2018 Published by Elsevier B.V.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序