Rapid and sensitive double-label based immunochromatographic assay for zearalenone detection in cereals

文献类型： 外文期刊

第一作者： Wang, Du

作者： Wang, Du;Zhang, Zhaowei;Zhang, Qi;Wang, Zhongzheng;Zhang, Wen;Yu, Li;Li, Hui;Jiang, Jun;Li, Peiwu;Zhang, Zhaowei;Zhang, Qi;Zhang, Wen;Li, Hui;Li, Peiwu;Zhang, Qi;Li, Peiwu;Jiang, Jun;Li, Peiwu;Wang, Du;Zhang, Wen;Yu, Li

作者机构：

关键词： Cereals; Double-label immunochromatographic assay; Quantitative; Zearalenone

期刊名称：ELECTROPHORESIS （ 影响因子：3.535； 五年影响因子：2.731 ）

ISSN： 0173-0835

年卷期： 2018 年 39 卷 16 期

页码：

收录情况： SCI

摘要： A double-label immunochromatographic based assay (DL-ICA) was developed to monitor zearalenone (ZEN) levels in cereals, based on Eu3+ nanoparticles (EuNP). The DL-ICA exhibited excellent sensitivity, reliability and selectivity in real samples. It showed low limits of detection (0.21-0.25 g/kg) and broad analytical ranges (up to 120 g/kg). The total analytical time, including sample preparation and DL-ICA execution, was reduced by 15 min compared with HPLC. The recovery rates ranged from 95.0-118.4%, with relative standard deviations (RSD) <11.6%. Inter- and intra-day validations were assessed, recovery rates of 89.3-106.9% and RSD of 2.3-9.7% were obtained, suggesting considerable stability and reliability for the assay. An excellent correlation was observed between DL-ICA and a reference HPLC method (R-2 = 0.9899). Compared to current immunoassays, the current DL-ICA is inexpensive, highly sensitive, and rapid. Therefore, DL-ICA constitutes a novel tool for monitoring mycotoxins in food and feed to ensure safety.

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