Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies

文献类型： 外文期刊

第一作者： Zhang, Lei

作者： Zhang, Lei;Cai, Xu;Wu, Jian;Cheng, Feng;Liang, Jianli;Cai, Chengcheng;Liu, Zhiyuan;Liu, Bo;Wang, Xiaowu;Zhang, Lei;Yang, Wencai;Liu, Min;Wang, Fan;Li, Song;Liu, Fuyan;Li, Xuming;Zheng, Hongkun;Grob, Stefan;Grossniklaus, Ueli;Cheng, Lin;Wang, Xiaowu;Li, Mai-he

作者机构：

期刊名称：HORTICULTURE RESEARCH （ 影响因子：6.793； 五年影响因子：6.589 ）

ISSN： 2052-7276

年卷期： 2018 年 5 卷

页码：

收录情况： SCI

摘要： Brassica rapa comprises several important cultivated vegetables and oil crops. Current reference genome assemblies of Brassica rapa are quite fragmented and not highly contiguous, thereby limiting extensive genetic and genomic analyses. Here, we report an improved assembly of the B. rapa genome (v3.0) using single-molecule sequencing, optical mapping, and chromosome conformation capture technologies (Hi-C). Relative to the previous reference genomes, our assembly features a contig N50 size of 1.45 Mb, representing a similar to 30-fold improvement. We also identified a new event that occurred in the B. rapa genome similar to 1.2 million years ago, when a long terminal repeat retrotransposon (LTR-RT) expanded. Further analysis refined the relationship of genome blocks and accurately located the centromeres in the B. rapa genome. The B. rapa genome v3.0 will serve as an important community resource for future genetic and genomic studies in B. rapa. This resource will facilitate breeding efforts in B. rapa, as well as comparative genomic analysis with other Brassica species.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序