Preparation, characterization, and evaluation of azoxystrobin nanosuspension produced by wet media milling

文献类型： 外文期刊

第一作者： Yao, Junwei

作者： Yao, Junwei;Cui, Bo;Zhao, Xiang;Wang, Yan;Zeng, Zhanghua;Sun, Changjiao;Yang, Dongsheng;Liu, Guoqiang;Cui, Haixin;Yao, Junwei;Gao, Jinming

作者机构：

关键词： Azoxystrobin; Nanosuspension; Antifungal activity; Fusarium oxysporum; Wet media milling

期刊名称：APPLIED NANOSCIENCE （ 影响因子：3.674； 五年影响因子：4.604 ）

ISSN： 2190-5509

年卷期： 2018 年 8 卷 3 期

页码：

收录情况： SCI

摘要： To improve the bioavailability of the poorly water-soluble fungicide, an azoxystrobin nanosuspension was prepared by the wet media milling method. Due to their reduced mean particle size and polydispersity index, 1-Dodecanesulfonic acid sodium salt and polyvinylpyrrolidone K30 were selected from six conventional surfactants, the content only accounting for 15% of the active compound. The mean particle size, polydispersity index, and zeta potential of the nanosuspension were determined to be 238.1 +/- 1.5 nm, 0.17 +/- 0.02 and -31.8 +/- 0.3 mV, respectively. The lyophilized nanosuspension mainly retained crystalline state, with only a little amorphous content as determined by powder X-ray diffraction. Compared to conventional fungicide formulations, the nanosuspension presented an increased retention volume and a reduced contact angle, indicating enhanced wettability and adhesion. In addition, the azoxystrobin nanosuspension showed the highest antifungal activity, with a medial lethal concentration of 1.4243 mu g/mL against Fusarium oxysporum. In optical micrographs, hyphal deformations of thinner and intertwined hyphae were detected in the exposed group. Compared to the control group, the total soluble protein content, superoxide dismutase, and catalase activities were initially increased and then decreased with prolonged exposure time. The azoxystrobin nanosuspension reduced the defensive antioxidant capability of Fusarium oxysporum and resulted in the generation of excessive reactive oxygen species. This study provides a novel method for preparing nanosuspension formulation of poorly soluble antifungal agents to enhance the biological activity and decrease the negative environmental impact.

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