Structural characterization of a bioactive water-soluble heteropolysaccharide from Nostoc sphaeroids kutz
文献类型： 外文期刊
第一作者： Liu, Yanfang
作者： Liu, Yanfang;Su, Panfeng;Zhang, Jingsong;Zhou, Shuai;Wang, Yatao;Tang, Qingjiu;Su, Panfeng;Xu, Jianfeng;Chen, Sheng;Wang, Yulan
作者机构：
关键词： Nostoc sphaeroids kutz; Polysaccharide; Structure; Conformation; Immunological activity; Macrophages; Activation
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）
ISSN： 0144-8617
年卷期： 2018 年 200 卷
页码：
收录情况： SCI
摘要： A water soluble Nostoc sphaeroids kutz polysaccharide (NSKP) was extracted and purified by precipitating hot-water extract from Nostoc sphaeroids kutz with 40% (v/v) ethanol. The yield was 40.10% (w/w), and weight average molecular weight (M-w) was determined as 1.31x10(5) g/mol. Cell culture study showed that NSKP could stimulate the NO release from RAW264.7 macrophages. The. value (R-g/R-h) of NSKP was calculated as 1.50, indicating flexible random coils conformation of NSKP in aqueous solution. According to methylation analysis, and 1D & 2D NMR spectra, the molecular structure of polysaccharide NSKP was deduced as below: [GRAPHICS] .
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