Detection of self-biting behavior of mink by loop-mediated isothermal amplification (LAMP) and sequence-characterized amplified regions (SCAR)

文献类型： 外文期刊

第一作者： Liu, Z. Y.

作者： Liu, Z. Y.;Song, S. S.;Huo, Z. S.;Song, X. C.;Cong, B.;Yang, F. H.

作者机构：

关键词： mink; self-biting; loop-mediated isothermal amplification; sequence-characterized amplified regions

期刊名称：POLISH JOURNAL OF VETERINARY SCIENCES （ 影响因子：0.821； 五年影响因子：1.005 ）

ISSN： 1505-1773

年卷期： 2018 年 21 卷 2 期

页码：

收录情况： SCI

摘要： Self-biting disease occurs in most farmed fur animals in the world. The mechanism and rapid detection method of this disease has not been reported. We applied bulked sergeant analysis (BSA) in combination with RAPD method to analyze a molecular genetic marker linked with self-biting trait in mink group. The molecular marker was converted into SCAR and loop-mediated isothermal amplification (LAMP) marker for rapid detection of this disease. A single RAPD marker A10 amplified a specific band of 1000bp in self-biting minks. The sequences of the bands exhibited 73% similarity to the Canis Brucella. SCAR and LAMP marker were designed for the specific fragment of RAPD marker A10 and validated in 30 self-biting minks and 30 healthy minks. chi(2 )test showed difference (p<0.05) with SCAR and significant difference (p<0.01) with LAMP in the detection rate between the two groups, but LAMP method was more accurate than SCAR method. This indicated that LAMP can be used as a positive marker to detect self-biting disease in minks.

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