Molecular characterization and expression analysis of strbp in Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Meng, Liang

作者： Meng, Liang;Xu, Wenteng;Zhu, Ying;Zhang, Ning;Shao, Changwei;Liu, Yang;Chen, Songlin;Meng, Liang;Xu, Wenteng;Zhu, Ying;Zhang, Ning;Shao, Changwei;Liu, Yang;Chen, Songlin

作者机构：

关键词： Cynoglossus semilaevis; Strbp; RNA-binding protein; Expression analysis; Spermatogenesis

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2018 年 118 卷

页码：

收录情况： SCI

摘要： Spermatid perinuclear RNA-binding protein (Strbp) is a kind of double-stranded (ds) RNA specific binding protein that plays important roles in mammalian spermatogenesis. In this study, we have isolated and characterized the strbp gene of Chinese tongue sole, Cynoglossus semilaevis, termed as CS-strbp. The CS-strbp genomic sequence contained fifteen exons and fourteen introns. Its cDNA was 2655 bp in length, encoding a 666-amino-acid protein with two conserved ds binding motifs. Using quantitative PCR, we found that CS-strbp mRNA exhibited sex-biased and tissue-specific distribution, predominantly expressed in the fertile male testis, though the expression levels varied throughout different developmental stages. Comparison of methylation profile in different sexual genotypes demonstrated the low methylation level of CS-strbp promoter in male and pseudo-male, which is consistent with the high expression levels in those genotypes. In situ hybridization revealed that CS-strbp mRNA mainly localized in male germ cells, especially in spermatids and spermatozoa. Given these findings, we postulate that CS-strbp might function in spermatogenesis of Chinese tongue sole. (C) 2018 Elsevier Inc. All rights reserved.

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