Identification and analysis of differentially expressed microRNAs in rainbow trout (Oncorhynchus mykiss) responding to infectious hematopoietic necrosis virus infection

文献类型： 外文期刊

第一作者： Cao, Yongsheng

作者： Cao, Yongsheng;Wang, Di;Li, Shaowu;Xu, Liming;Zhao, Jingzhuang;Liu, Hongbai;Lu, Tongyan;Cao, Yongsheng;Zhang, Qiya

作者机构：

关键词： microRNA; Rainbow trout; IHNV; Deep sequencing; Functional analysis

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2018 年 88 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are a class of regulators essential for numerous biological processes. Infectious hematopoietic necrosis virus (IHNV) is one of the most important viral pathogens in salmon and trout. In this study, the miRNA expression profiles of rainbow trout upon IHNV infection were explored. In total, 392 known miRNAs and 936 novel miRNAs were identified. Twelve known and 13 novel miRNAs were differentially expressed between infected and uninfected fish. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that certain miRNA target genes were associated with biological regulation, the immune system, and signal transduction. In addition, over- and suppressed expression of miR-146a-3p, miR-155-5p, miR-216a-5p, and miR-499b-5p could respectively increase and decrease viral gene expression in cells and viral titers. MiR-146a-3p and miR-216a-5p inhibited the expression of type-I IFN and the Mxl gene induced by IHNV. These results provide preliminary insights into the IHNV-host interactions mediated by miRNAs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序