Involvement of a short-type peptidoglycan recognition protein (PGRP) from Chinese giant salamanders Andrias davidianus in the immune response against bacterial infection

文献类型： 外文期刊

第一作者： Yang, Hui

作者： Yang, Hui;Li, Xixi;Ji, Jiaojun;Zhang, Yingying;Zhang, Xiaojun;Song, Weijia;Wang, Lixin;Li, Fenggang

作者机构：

关键词： Andrias davidianus; PGRP-SC2; PRR; Immune response

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2018 年 88 卷

页码：

收录情况： SCI

摘要： PGRPs (Peptidoglycan recognition proteins) could recognize peptidoglycan and play vital roles in innate immunity among different animals. Till present, the functions of PGRP have been studied in various animals, but few reports have studied the amphibian PGRPs. In the current research, a short type PGRP was identified from Chinese giant salamander and its involvement in the innate immunity was studied. The ORF of AdPGRP-SC2 cDNA was 573 bp, which encoded 190 amino acids, and contained a PGRP and an amidase_2 domain. The qPCR analysis revealed that AdPGRP-SC2 mRNA transcripts expressed in different tissues, with the highest expression level in muscle, intestine and spleen. Results of immune challenges with peptidoglycan (PGN) demonstrated that expression patterns of AdPGRP-SC2 were significantly up-regulated in erythrocyte and spleen at the early injection stage. The recombinant AdPGRP-SC2 protein was successfully produced and purified, and it could show binding affinity to different bacteria. In the presence of Zn2+, the rAdPGRP-SC2 could exhibit a broad PAMPs binding activities, strongly agglutinate bacteria and exhibit amidase enzyme activity. Collectively, these data indicate AdPGRP-SC2 could act as PRR to recognize the invading microorganisms and as the antimicrobial effectors during the innate immune response of A. davidianus.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序