Variation in Ploidy Level and Morphological Traits in the Progeny of the Triploid Apple Variety Jonagold

文献类型： 外文期刊

第一作者： He, Ping

作者： He, Ping;Li, Linguang;Cheng, Lailiang;Wang, Haibo;Chang, Yuansheng

作者机构：

关键词： flow cytometry; karyotypic analysis; S-alleles; SSR markers

期刊名称：CZECH JOURNAL OF GENETICS AND PLANT BREEDING （ 影响因子：0.865； 五年影响因子：1.017 ）

ISSN： 1212-1975

年卷期： 2018 年 54 卷 3 期

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收录情况： SCI

摘要： Variation with respect to both ploidy level and morphology was characterized for a set of 690 seedlings of the triploid apple variety Jonagold, of which 481 were obtained via in vitro culture of mature embryos, and 209 via conventional germination. Their ploidy level was determined by a combination of flow cytometry and root tip chromosome counting. The assessed morphological traits were leaf length, width and shape, stomatal density, guard cell length and chloroplast width and number. A total of 452 seedlings were aneuploid, 225 diploid, nine triploid and four tetraploid. All four tetraploid seedlings were derived by in vitro culture. When the triploid seedlings were genotyped at the S-locus and at selected microsatellite loci, we found that the Jonagold stigma was compatible with pollen which shared some of the maternal parent S-locus alleles.

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