In vitro culture and biological properties of broiler adipose-derived stem cells

文献类型： 外文期刊

第一作者： Lu, Tengfei

作者： Lu, Tengfei;Pei, Wenhua;Wu, Yangnan;Guan, Weijun;Wang, Kunfu;Zhang, Shuang;Chen, Fenghao

作者机构：

关键词： adipose-derived stem cells; broiler; in vitro culture; biological properties; differentiation

期刊名称：EXPERIMENTAL AND THERAPEUTIC MEDICINE （ 影响因子：2.447； 五年影响因子：2.263 ）

ISSN： 1792-0981

年卷期： 2018 年 16 卷 3 期

页码：

收录情况： SCI

摘要： In the past 10 years, adipose-derived stem cells (ADSCs) have been applied due to their pluripotency. Experimental tissues have been frequently obtained from mammals, including rabbits, mice and humans, but rarely from broilers, Gallus gallus domesticus. In the present study, ADSCs were obtained from 20-day-old broiler embryos. Primary ADSCs were sub-cultured to passage 37 in vitro. The surface markers of ADSCs, namely CD29, CD31, CD44, CD71 and CD73, were detected by reverse transcription polymerase chain reaction and immunofluorescence assays. The result indicated that CD29, CD44, CD71 and CD73 were expressed on the surface of cells at various passages, but not CD31. The growth curve of cells at the different passages had a typical sigmoidal shape. Furthermore, ADSCs were successfully induced to differentiate into osteoblasts, adipocytes and hepatocyte-like cells. The results denote that the ADSCs isolated from broilers have similar biological properties to those of ADSCs obtained from other animals. The present study provided a theoretical and experimental foundation for the use of poultry as a source of stem cells, and laid a foundation for adipose tissue engineering and strategies in regenerative medicine.

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