Corticosterone induces growth hormone expression in pituitary somatotrophs during goose embryonic development

文献类型： 外文期刊

第一作者： Yu, Jianning

作者： Yu, Jianning;Yan, Leyan;Chen, Zhe;Li, Hui;Zhu, Huanxi;Chen, Rong;Shi, ZhenDan;Yu, Jianning;Yan, Leyan;Chen, Zhe;Li, Hui;Zhu, Huanxi;Chen, Rong;Shi, ZhenDan

作者机构：

关键词： Corticosterone; Goose embryonic development; Growth hormone; Pituitary; Somatotroph

期刊名称：JOURNAL OF REPRODUCTION AND DEVELOPMENT （ 影响因子：2.214； 五年影响因子：2.221 ）

ISSN： 0916-8818

年卷期： 2018 年 64 卷 4 期

页码：

收录情况： SCI

摘要： Treatment of fetal rat and embryonic chicken with exogenous glucocorticoids induces premature differentiation of growth hormone (GH) secreting cells. The effect of corticosterone (CORT) on somatotroph differentiation was mostly studied in pituitary cells in vitro. Currently, there is no evidence for glucocorticoid-mediated induction of somatotroph differentiation during pituitary development in bird species other than chicken. In this study, we sought to find out if in ovo injection of corticosterone into developing goose embryos could induce premature increase of GH in somatotrophs. On embryonic day (e) 15, the albumen of fertile goose eggs was injected with 300 mu l of 0.9% saline, 300 mu l 5 x 10(-8) M CORT, or 300 mu l 5 x 10(-6) M CORT. Embryos were allowed to develop until e20 and e28 and isolated pituitaries were subjected to quantitative real-time PCR and immunocytochemistry to detect GH mRNA and protein, respectively. At e20 and e28, blood from chorioallantoic vessels was subjected to radioimmunoassay for analysis of plasma GH protein. In ovo administration of exogenous corticosterone brought about a 2.5-fold increase in the expression of GH mRNA and increased the in situ expression of GH protein in goose pituitary cells, and enhanced plasma GH levels compared to that of the respective controls at e20. These findings prove that administration of glucocorticoid could stimulate the expression of GH in somatotrophs during goose embryonic development. Our results suggest the probable involvement of membrane glucocorticoid receptor in the corticosterone mediated expression of GH. Together with previously published data, our results suggest that corticosterone mediated induction of GH expression during embryonic development is relatively conserved among different vertebrates.

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