文献类型： 外文期刊

第一作者： He Xiao-hong

作者： He Xiao-hong;Chen Xiao-fei;Pu Ya-bin;Guan Wei-jun;Song Shen;Zhao Qian-jun;Li Xiang-chen;Jiang Lin;Ma Yue-hui;He Xiao-hong;Chen Xiao-fei;Pu Ya-bin;Guan Wei-jun;Song Shen;Zhao Qian-jun;Li Xiang-chen;Jiang Lin;Ma Yue-hui;Chen Xiao-fei

作者机构：

关键词： scurs; sheep; iTRAQ; horn development; ECM-receptor interaction pathway

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 8 期

页码：

收录情况： SCI

摘要： Scurs is a horn phenotype that exhibits as small corneous structures on the skull due to the deformed development of horn tissues. Previous genome-wide association analysis of scurs in Soay sheep showed a significant association to the polled locus, relaxin-like receptor 2 (RXFP2). However, the molecular mechanism underlying the development of scurs remains largely unknown. In the present study, we performed an iTRAQ-based quantitative proteomic analysis of horn tissues from both scurs and normal two-horned and four-horned individuals among Altay sheep to identify the differentially expressed proteins (DEPs) responsible for the scurs phenotype. In total, 232 proteins showed significant differential expression, and the most significant Gene ontology categories were the adhesion processes (biological adhesion (P=4.07x 10(-17)) and cell adhesion (P=3.7x 10(-16))), multicellular organismal process (single-multicellular organism process (P=2.06x10(-11)) and multicellular organismal process (P=2.29 x10(-11))) and extracellular processes (extracellular matrix organization (P=4.77x 10(-16)) and extracellular structure organization (P=4.93 x 10(-16))). Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that extracellular matrix (ECM)-receptor interactions and focal adhesion pathways were the most significant pathways. This finding is consistent with the reduced formation of extracellular matrix in scurs and the development of deformed horn tissues. Our study helps to elucidate the inheritance pattern of sheep horn traits from the perspectives of downstream expressed proteins.

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