Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense

文献类型： 外文期刊

第一作者： Li, Ting-Gang

作者： Li, Ting-Gang;Zhang, Dan-Dan;Zhou, Lei;Kong, Zhi-Qiang;Hussaini, Adamu S.;Wang, Dan;Li, Jun-Jiao;Wang, Bao-Li;Yin, Chun-Mei;Chen, Jie-Yin;Dai, Xiao-Feng;Short, Dylan P. G.;Dhar, Nikhilesh;Subbarao, Krishna V.;Short, Dylan P. G.;Dhar, Nikhilesh;Subbarao, Krishna V.;Klosterman, Steven J.

作者机构：

关键词： cotton; Verticillium wilt; cysteine-rich receptor-like kinases (CRKs); expression profiling; defense response

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticillium dahliae that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical CRKs in a Gossypium barbadense genome (GbCRKs). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the GbCRKs could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the GbCRKs in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the GbCRK genes were up-regulated in response to V. dahliae infection. Virus-induced gene silencing of each of these nine GbCRKs independently revealed that the silencing of GbCRK18 was sufficient to compromise VW resistance in G. barbadense. GbCRK18 expression could be induced by V. dahliae infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the CRK gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that GbCRK18 in particular confers VW resistance in G. barbadense.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序