Discovery of Plant Viruses From Tea Plant (Camellia sinensis (L.) O. Kuntze) by Metagenomic Sequencing
文献类型： 外文期刊
第一作者： Hao, Xinyuan
作者： Hao, Xinyuan;Zhang, Weifu;Liu, Ying;Qian, Wenjun;Wang, Yuchun;Wang, Lu;Zeng, Jianming;Yang, Yajun;Wang, Xinchao;Hao, Xinyuan;Zhang, Weifu;Liu, Ying;Qian, Wenjun;Wang, Yuchun;Wang, Lu;Zeng, Jianming;Yang, Yajun;Wang, Xinchao;Zhao, Fumei
作者机构：
关键词： NGS; virus diversity; virus discovery; plant disease; leaf discoloration
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2018 年 9 卷
页码：
收录情况： SCI
摘要： The tea plant (Camellia sinensis (L.) O. Kuntze) is an economically important woody species. In this study, we collected 26 tea plant samples with typical discoloration symptoms from different tea gardens and performed metagenomic analysis based on next-generation sequencing. Homology annotation and PCR sequencing validation finally identified seven kinds of plant viruses from tea plant. Based on abundance distribution analysis, the two most abundant plant viruses were highlighted. Genetic characterization suggested that they are two novel virus species with relatively high homology to Blueberry necrotic ring blotch virus and American plum line pattern virus. We named the newly discovered viruses tea plant necrotic ring blotch virus (TPNRBV) and tea plant line pattern virus (TPLPV). Evolutionary relationship analysis indicated that TPNRBV and TPLPV should be grouped into the Blunervirus and the Ilarvirus genera, respectively. TPLPV might have same genome activation process with known ilarviruses based on sequence analysis. Moreover, specific primers for both viruses detection were designed and validated. The symptoms and ultrastructure of TPNRBV infected leaves were first recorded. Virus detections in the symptomatic and asymptomatic tissues from field plants showing tea plant necrotic ring blotch disease suggest that TPNRBV has a systemic movement feature. In summary, we first identified seven kinds of putative plant viruses by metagenomic analysis and report two novel viruses being latent pathogens to tea plant. The results will advance our understanding of tea plant virology and have significance for the genetic breeding of tea plants in the future.
分类号：
- 相关文献
作者其他论文 更多>>
-
Synergistic antimicrobial efficacy of glabrol and colistin through micelle-based co-delivery against multidrug-resistant bacterial pathogens
作者：Liu, Ying;Zhang, Mengying;Qu, Shaoqi;Liu, Ying;Mei, Chen;Wang, Hongjun;Cai, Yawei;Wu, Shuaicheng
关键词：Gram-negative bacteria; Colistin; Glabrol; Synergistic antibacterial; Micelle
-
Revealing the Molecular Regulatory Mechanism of Flavonoid Accumulation in Tender Leaves of Tea Plants by Transcriptomic and Metabolomic Analyses
作者：Shan, Ruiyang;You, Xiaomei;Kong, Xiangrui;Zhang, Yazhen;Li, Xinlei;Chen, Changsong;Zhang, Yongheng;Wang, Lu;Wang, Xinchao
关键词：
Camellia sinensis ; transcriptomics; flavonoids; regulatory network -
Interactions, properties and lipid digestibility of attractive Pickering emulgels formed by sequential addition of oppositely charged nanopolysaccharides
作者：Guo, Shasha;Ji, Xingxiang;Guo, Shasha;Li, Jun;Huang, Luyao;Guo, Shasha;Wan, Zhangmin;Niu, Xun;Xu, Junhua;Liu, Ying;Bai, Long;Lu, Yi;Rojas, Orlando J.;Jiao, Wenjuan;Zheng, Jianan;Li, Bin;Bai, Long;Lu, Yi;Rojas, Orlando J.;Rojas, Orlando J.
关键词：
-
Structural characterization and hypolipidemic activity of a hetero-galactan purified from Sanghuangporus vaninii based on modulation of TLR4/NF-κB pathway
作者：Hao, Jie;Zhu, Yanfeng;Li, Zhige;Wang, Lu;Qu, Yidi;Wang, Di;Zhang, Yongfeng;Li, Lanzhou;Wang, Di;Yu, Hailong;Qi, Liangliang
关键词：Sanghuangporus vaninii; Polysaccharide; Hypolipidemic efficacy; Inflammation; TLR4/NF-kappa B
-
Two leucine-rich repeat receptor-like kinases initiate herbivory defense responses in tea plants
作者：Jiang, Qi;Ding, Changqing;Feng, Lingjia;Wu, Zhenwei;Liu, Yujie;He, Lintong;Liu, Chuande;Wang, Lu;Zeng, Jianming;Huang, Jianyan;Ye, Meng
关键词：
-
CsCIPK20 Improves Tea Plant Cold Tolerance by Modulating Ascorbic Acid Synthesis Through Attenuation of CsCSN5-CsVTC1 Interaction
作者：Di, Taimei;Wu, Yedie;Wang, Jie;He, Mingming;Huang, Jianyan;Li, Nana;Hao, Xinyuan;Ding, Changqing;Zeng, Jianming;Yang, Yajun;Wang, Xinchao;Wang, Lu
关键词：ascorbic acid; CsCIPK20; CsVTC1; low temperature
-
Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum
作者：Zhi, Yan;Mei, Chen;Liu, Zhenyi;Liu, Ying;Wang, Hongjun
关键词：Glutathione reductase;
Av. paragallinarum ; Oxidative stress; Bacterial virulence; Redox homeostasis; Metabolic adaptation
