Stress resistance, motility and biofilm formation mediated by a 25kb plasmid pLMSZO8 in Listeria monocytogenes

文献类型： 外文期刊

第一作者： Zhang, Hui

作者： Zhang, Hui;Wu, Litin;Zhu, Mengya;Bao, Hongduo;Zhou, Yan;Pang, Maoda;Wang, Ran;Hu, Yunhe;Zhou, Chenglu;Yang, Zhenquan;Zhu, Mengya;Zhou, Chenglu;Yang, Zhenquan;Zhu, Mengya;Zhou, Xiaohui;Zhou, Chenglu;Yang, Zhenquan;Zhu, Mengya;Zhou, Xiaohui;Zhou, Chenglu;Yang, Zhenquan;Zhu, Mengya;Zhou, Xiaohui;Zhou, Xiaohui

作者机构：

关键词： Listeria monocytogenes; Plasmid; Biofilm; Motility

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2018 年 94 卷

页码：

收录情况： SCI

摘要： Listeria monocytogenes is a foodbome pathogen that can form biofilm and tolerate severe environment condition and thus persist in food product for a prolonged period, which poses a significant risk to food safety. While some chromosomal genetic determinants associated with stress resistance and biofilm formation have been characterized, plasmid-mediated stress resistance in particular L. monocytogenes strains are less known. In this study, we showed a L. monocytogenes strain SZO8 (serovar 1/2b) isolated from a raw meat processing environment harbored a 25kb plasmid, designated as pLMSZ08. After incubation under the selective condition containing novobiocin, pLMSZO8 was successfully cured from SZ08. Compared to its parental strain SZ08, the cured strain SZO8* was not able to grow well under the high concentration of sodium chloride (5.5%). SZO8* displayed higher efficiency of plaque formation compared with its parental strain SZ08. There were no significant growth difference under different temperature and pH conditions between SZO8 and SZ08*. Biofilm formation of SZO8* reduced significantly compared with SZO8 after 48hr incubation. Furthermore, the relative expression levels of flagella -associated genes in SZO8* were up-regulated comparing to those in SZ08. We have sequenced the pLMSZO8 and the sequencing results showed that pLMSZO8 harbors genes associated with transcriptional regulation, metal/drug transport, histidine kinase and ATPase. These results suggested that these genes encoded by pLMSZO8 are associated with tolerance, motility and biofihn formation in L. monocytogenes SZ08.

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