文献类型： 外文期刊

第一作者： Du, Gai Mei

作者： Du, Gai Mei;Luo, Bi Ping;Hu, Zhi Hua;Wu, Jie Ge;Yan, Wen Mei;Han, Zheng Qiang;Zhang, Yu Hong;Liu, Mao Jun;Liu, Mao Jun

作者机构：

关键词： GOAT; H+-K+-ATPase activity; Acylated ghrelin; Total ghrelin

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

ISSN： 0016-6480

年卷期： 2018 年 267 卷

页码：

收录情况： SCI

摘要： Ghrelin is implicated in the regulation of gastric functional development. The octanoylation of ghrelin is critical for its physiological functions which dependent upon ghrelin O-acyltransferase (GOAT) catalyzation. To investigate the effect of GOAT on gastric acid secretion and expression of ghrelin in vitro. Primary cultures of gastric mucosal cells were challenged with 1.5 x 10(-5), 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL GO-CoA-Tat (The GOAT inhibitor), respectively, for 24 h in order to further clarify the effect of GOAT on H+-K+-ATPase activity. In vitro, GO-CoA-Tat significantly increased ghrelin and GOAT mRNA expression at 1.5 x 10(-5), 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL, and augmented cell total ghrelin secretion at 1.5 x 10(-3) mol/mL. But cell acylated ghrelin secretion was reduced at 1.5 x 10(-3) mol/mL GO-CoA-Tat (P < 0.05). And cell acylated ghrelin synthesis was reduced at 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL GO-CoA-Tat (P < 0.05). In accordance with acylated ghrelin level, H+-K+-ATPase activity were decreased with 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL GO-CoA-Tat (P < 0.05). These results indicated that GOAT inhibitor decreases the acylated ghrelin level and H+-K+-ATPase activity in vitro.

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