Immunization with a suicidal DNA vaccine expressing the E glycoprotein protects ducklings against duck Tembusu virus

文献类型： 外文期刊

第一作者： Tang, Jingyu

作者： Tang, Jingyu;Bi, Zhuangli;Yin, Dongdong;Zhang, Li;Zhu, Yingqi;Wang, Guijun;Tang, Jingyu;Bi, Zhuangli;Ding, Mingyang;Zhu, Jie;Zhang, Li;Miao, Qiuhong;Zhu, Yingqi;Liu, Guangqing;Yin, Dongdong;Wang, Guijun

作者机构：

关键词： Suicidal DNA vaccine; Duck tembusu virus; E glycoprotein; Ducklings

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2018 年 15 卷

页码：

收录情况： SCI

摘要： Backgroud: Duck Tembusu virus (DTMUV), a pathogenic flavivirus, emerged in China since 2010 and causing huge economic loss in the Chinese poultry industry. Although several vaccines have been reported to control DTMUV disease, few effective vaccines are available and new outbreaks were continuously reported. Thus, it is urgently to develop a new effective vaccine for prevention of this disease. Methods: In this study, a suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon and DTMUV E glycoprotein gene was constructed and the efficacy of this new vaccine was assessed according to humoral and cell-mediated immune responses as well as protection against the DTMUV challenge in ducklings. Results: Our results showed that the recombinant SFV replicon highly expressed E glycoprotein in DEF cells. After intramuscular injection of this new DNA vaccine in ducklings, robust humoral and cellular immune responses were observed in all immunized ducklings. Moreover, all ducklings were protected against challenge with the virulent DTMUV AH-F10 strain. Conclusions: In conclusion, we demonstrate that this suicidal DNA vaccine is a promising candidate facilitating the prevention of DTMUV infection.

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