Development of a TaqMan probe-based quantitative reverse transcription PCR assay for detection of Getah virus RNA
文献类型： 外文期刊
第一作者： Shi, Ning
作者： Shi, Ning;Hu, Bo;Zhang, Lei;Zhao, Chuan-fang;Deng, Xiao-yu;Li, Xin-tong;Xue, Xiang-hong;Bai, Xue;Zhang, Hai-ling;Lu, Rong-guang;Lian, Shi-zhen;Wang, Yang;Yan, Xi-jun;Li, Li-xia;Yan, Ming-hao;Liu, Hao
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2018 年 163 卷 10 期
页码：
收录情况： SCI
摘要： Getah virus (GETV), a mosquito-borne virus that mainly infects horses and pigs, has emerged and spread in China. We developed a highly specific and reproducible TaqMan probe-based quantitative reverse transcription PCR (RT-qPCR) assay targeting the non-structural protein 1 of GETV, whose detection limit is 25.5 copies/A mu L, which is 100-fold higher than that of conventional RT-PCR. RT-qPCR was used to detect GETV RNA in mosquito and animal clinical samples, showing that the accuracy of RT-qPCR was higher than that of conventional RT-PCR. The newly developed RT-qPCR assay may be a useful alternative tool for rapid, simple and specific diagnosis of GETV infection.
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