Direct from solid natural products to pure compounds in a single step: Coupling online extraction with high-speed counter-current chromatography

文献类型： 外文期刊

第一作者： Guo, Keke

作者： Guo, Keke;Peng, Mijun;Shi, Shuyun;Guo, Keke;Tong, Chaoying;Shi, Shuyun;Peng, Mijun;Liu, Yuge

作者机构：

关键词： alkaloids; high-speed counter-current chromatography; natural products; online extraction; phenolics

期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）

ISSN： 1615-9306

年卷期： 2018 年 41 卷 18 期

页码：

收录情况： SCI

摘要： Extraction is the most important step in the purification of bioactive compounds from natural products. This study introduces a simple online extraction strategy coupled with high-speed counter-current chromatography for efficient extraction and purification of bioactive components from solid natural products. For online extraction strategy, 1.0g of ground Mangnolia officinalis or Piper nigrum was loaded into a guard column, which was then positioned on the manual injection valve instead of the sample loop. Bioactive components were directly extracted by the mobile phase of high-speed counter-current chromatography, and then transferred into high-speed counter-current chromatography for purification. In addition, the compatibility of the developed methodology for direct purification of bioactive components from fresh M. officinalis was successfully demonstrated. Obviously, in comparison with traditional offline heat-reflux extraction, online extraction avoided the instrument, time, solvent, and energy consumption, and purified two phenolic compounds (honokiol and magnolol) from M. officinalis and three alkaloids (piperyline, piperine, and piperanine) from P. nigrum with high extraction efficiency. The superiority of the developed methodology is to establish an easy, rapid, and efficient technique for the purification of a wide variety of bioactive components from solid natural products.

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