Hoeflea prorocentri sp nov., isolated from a culture of the marine dinoflagellate Prorocentrum mexicanum PM01

文献类型： 外文期刊

第一作者： Yang, Qiao

作者： Yang, Qiao;Jiang, Zhi-Wei;Huang, Cheng-Hui;Feng, Li-Juan;Yang, Guang-Feng;Zhang, Xiao-Ling;Mu, Jun;Zhang, Ruo-Nan;Li, Ling-Zhi;Zhang, Heng;Yang, Guang-Feng

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关键词： Hoeflea prorocentri sp; nov; Novel species; Prorocentrum mexicanum; Algal-associated bacterium

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

年卷期： 2018 年 111 卷 10 期

页码：

收录情况： SCI

摘要： A Gram-stain negative, aerobic, rod-shaped, non-motile, yellow-pigmented and non-spore-forming bacterial strain, designated PM5-8(T), was isolated from a culture of a marine toxigenic dinoflagellate Prorocentrum mexicanum PM01. Strain PM5-8(T) grew at 15-35 degrees C (optimum, 25-30 degrees C) and pH 6-11 (optimum, 7.5-8). Cells required at least 1.5% (w/v) NaCl for growth, and can tolerate up to 7.0% with the optimum of 4%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain PM5-8(T) is closely related to members of the genus Hoeflea, with high sequence similarities with Hoeflea halophila JG120-1(T) (97.06%) and Hoeflea alexandrii AM1V30(T) (97.01%). DNA-DNA hybridization values between the isolate and other type strains of recognized species of the genus Hoeflea were between 11.8 and 25.2%, which is far below the value of 70% thresholdfor species delineation. The DNA G+C content was 50.3mol%. The predominant cellular fatty acids of the strain wereidentified assummed feature 8 (C(16:1)7c and/or C(16:1)6c; 51.5%), C(18:1)7c 11-methyl (20.7%), C-16:0 (17.2%) and C-18:0 (5.7%). The major respiratory quinone was Q-10. Polar lipids profiles contained phosphatidylcholine, phosphatidylglycerol, sulfoquinovosyl diacylglycerol, phosphatidylmono- methylethanolamine, phosphatidylethanolamine and four unidentified lipids. On the basis of the polyphasic taxonomic datapresented, strain PM5-8(T) (=CCTCC AB 2016294(T)=KCTC 62490(T)) represents a novel species of the genus Hoeflea, for which the name Hoeflea prorocentri sp. nov. is proposed.

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