Morphological and molecular identification of chalkbrood disease pathogen Ascosphaera apis in Apis cerana cerana

文献类型： 外文期刊

第一作者： Chen, Dafu

作者： Chen, Dafu;Guo, Rui;Xiong, Culling;Zheng, Yanzhen;Fu, Zhongming;Hou, Chunsheng

作者机构：

关键词： chalkbrood; Ascosphaera apis; Apis cerana cerana; drone; molecular identification

期刊名称：JOURNAL OF APICULTURAL RESEARCH （ 影响因子：2.584； 五年影响因子：2.52 ）

ISSN： 0021-8839

年卷期： 2018 年 57 卷 4 期

页码：

收录情况： SCI

摘要： Chalkbrood is a fungal disease of honey bees and leads to serious apicultural loss worldwide. Ascosphaera apis, the pathogen of chalkbrood, has been found in Apis mellifera, Apis cerana, Xylocopa californica arizonensis larvae as well as adult bumble bees. Here, the fungal pathogen of Apis cerana cerana drone mummies was isolated and identified using morphological and molecular methods. Morphological observation indicated that the sizes of the fruiting bodies, spore balls and ascospores of the fungus isolated from A. c. cerana drone mummies were similar to those of A. apis. Phylogenetic analysis suggested that the fungus is identical to A. apis. Furthermore, the results of a cross infection assay demonstrated that the isolated fungus is capable of infecting eastern and western honey bee larvae and results in chalkbrood. These results confirmed that the isolated fungal pathogen is A. apis. This is the first documentation of morphological and molecular identification of A. apis in A. c. cerana. Our results not only provide novel insight to better understand the pathology of A. apis, but also lay a solid foundation for further investigations of host responses and pathogen-host interactions during chalkbrood of eastern honey bee larvae.

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