Identification and Functional Analysis of Two New Mutant BnFAD2 Alleles That Confer Elevated Oleic Acid Content in Rapeseed
文献类型： 外文期刊
第一作者： Long, Weihua
作者： Long, Weihua;Hu, Maolong;Gao, Jianqin;Chen, Song;Zhang, Jiefu;Cheng, Li;Pu, Huiming
作者机构：
关键词： rapeseed (Brassica napus L.); BnFAD2; single-nucleotide polymorphism; oleic acid; homolog genes; genotyping
期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）
ISSN： 1664-8021
年卷期： 2018 年 9 卷
页码：
收录情况： SCI
摘要： Rapeseed (Brassica napus L.) is a vital oil crop worldwide. High oleic acid content is a desirable quality trait for rapeseed oil, which makes it more beneficial to human health. However, many germplasm resources with high oleic acid content in rapeseed have not been evaluated with regard to their genotypes, making it difficult to select the best strains with this trait for the breeding of high oleic acid rapeseed variety. This work was to explore the gene-regulation mechanism of this trait using a new super-high oleic acid content (similar to 85%) line N1379T as genetic material. In this study, the sequences of four homologous fatty acid desaturase (BnFAD2) genes were compared between super-high (similar to 85%, N1379T) and normal (similar to 63%) oleic acid content lines. Results showed that there were two single-nucleotide polymorphisms (SNPs) in BnFAD2-1 and BnFAD2-2, respectively, which led to the amino acid changes (E106K and G303E) in the corresponding proteins. Functional analysis of both genes in yeast confirmed that these SNPs were loss-of-function mutations, thus limiting the conversion of oleic acid to linoleic acid and resulting in the considerable accumulation of oleic acid. Moreover, two specific cleaved amplified polymorphic sequences (CAPS) markers for the two SNPs were developed to identify genotypes of each line in the F-2 and BC1 populations. Furthermore, these two mutant loci of BnFAD2-1 and BnFAD2-2 genes were positively associated with elevated oleic acid levels and had a similar effect with regard to the increase of oleic acid content. Taken together, these two novel SNPs in two different BnFAD2 genes jointly regulated the high oleic acid trait in this special germplasm. The study provided insight into the genetic regulation involved in oleic acid accumulation and highlighted the use of new alleles of BnFAD2-1 and BnFAD2-2 in breeding high oleic acid rapeseed varieties.
分类号：
- 相关文献
作者其他论文 更多>>
-
Improving
d -Allulose Production via Alditol Oxidase-Directed Evolution Assisted by an Artificial Genetic Circuit作者：Lv, Yongkun;Chen, Song;Zhu, Lijuan;Wang, Shilei;Shi, Mengzhuo;Xu, Jingliang;Lv, Yongkun;Guo, Zhouyan;Xu, Peng;Chen, Zhenhua;Zhao, Anqi;Wang, Weigao;Xu, Yameng;Xu, Peng
关键词：laboratory-directed evolution; D-allulose; alditol oxidase; artificial genetic circuit; growthcoupling
-
Comparative Analyses of Gut Microbiomes in Hycleus cichorii (Coleoptera: Meloidae) Adults Reveal Their Distinct Microbes, Microbial Diversity and Composition Associated to Food
作者：Yi, Chunyan;Zhang, Cuicui;Wang, Yanping;Liu, Xu;Chen, Song;Gao, Li;Du, Chao;Yang, Yongli
关键词：feeding habits; food; full-length 16S rDNA; gut microbiota
-
Optimized straw incorporation depth can improve the nitrogen uptake and yield of rapeseed by promoting fine root development
作者：Wang, Chunyun;Wang, Zongkai;Lou, Hongxiang;Wang, Xianling;Shao, Dongli;Tan, Xiaoqiang;Liu, Mengzhen;Wang, Bo;Kuai, Jie;Wang, Jing;Xu, Zhenghua;Zhou, Guangsheng;Zhao, Jie;Wang, Chunyun;Gao, Jianqin;Zhang, Jiefu
关键词：Straw incorporation; Rapeseed; Nitrogen; Root; Yield
-
Study on the influence mechanism of raw potato flour on the stability of ketchup
作者：Fu, Boqing;Fang, Jiahui;Gu, Zhengbiao;Hong, Yan;Cheng, Li;Fu, Boqing;Wang, Xu;Gu, Zhengbiao;Hong, Yan;Cheng, Li
关键词：Non-starch polysaccharides; Tomato; Rheology; Viscoelasticity; Water competition; Stability
-
Optimized tillage regimes in a rice-oilseed rape rotation system enhance system productivity by delaying post-flowering senescence
作者：Wang, Chunyun;Wang, Zongkai;Lou, Hongxiang;Wang, Xianling;Tan, Xiaoqiang;Shao, Dongli;Liu, Mengzhen;Wang, Bo;Kuai, Jie;Wang, Jing;Xu, Zhenghua;Zhou, Guangsheng;Zhao, Jie;Wang, Chunyun;Gao, Jianqin;Zhang, Jiefu
关键词：Moderate deep tillage; Oilseed rape; Rice; Root; Yield
-
The Aldehyde Dehydrogenase Superfamily in Brassica napus L.: Genome-Wide Identification and Expression Analysis Under Low-Temperature Conditions
作者：Jin, Ting;Ding, Chao;Long, Weihua;Wu, Chunhua;Huang, Zhen;Zhang, Xingguo;Li, Shimeng
关键词：aldehyde dehydrogenase;
Brassica napus L.; cold stress; expression analysis; natural variation -
Integrating QTL mapping and GWAS to decipher the genetic mechanisms behind the calcium contents of Brassica napus shoots
作者：Xiang, Yanan;Wang, Xiaodong;Zhang, Jiefu;Xiang, Yanan;Chen, Feng;Shi, Rui;Yang, Tinghai;Zhang, Wei;Zhou, Xiaoying;Wang, Chunyun;Sun, Chengming;Fu, Sanxiong;Wang, Xiaodong;Zhang, Jiefu;Shen, Yue
关键词：
Brassica napus shoots; calcium concentration; QTL mapping; GWAS; candidate genes
