Exploring the TLR and NLR signaling pathway relevant molecules induced by the Theileria annulata infection in calves

文献类型： 外文期刊

第一作者： Yin, Fangyuan

作者： Yin, Fangyuan;Liu, Junlong;Gao, Shandian;Liu, Aihong;Zhao, Shuaiyang;Li, Sitong;Wang, Jinming;Li, Youquan;Luo, Jianxun;Guan, Guiquan;Yin, Hong;Yin, Hong

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关键词： Theileria annulata; Innate immunity; TLRs; NLRs; PCR array

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2018 年 117 卷 10 期

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收录情况： SCI

摘要： Theileria annulata is the pathogen of bovine tropical theileriosis. It is extremely harmful to the cattle industry, with huge economic losses. The toll-like receptor (TLR) and NOD-like receptor (NLR) signaling pathways are crucial for resistance to infection of the protozoa, such as Plasmodium falciparum, Toxoplasma gondii, and Trypanosoma cruzi. However, the role of these immune-related pathways is unclear during T. annulata infection. In the present study, peripheral blood mononuclear cells and serum were separated from blood samples of calves infected with homogenized tick supernatants carrying T. annulata sporozoites at 12 h, 24 h, 36 h, 48 h, 72 h, 96 h, 120 h, 144 h and 168 h postinoculation. The Custom RT2 Profiler PCR Array was used to explore the mRNA levels of 42 TLR and NLR signaling pathway relevant genes. The TLR1, TLR6, TLR10, NLRP1, and MyD88 genes and their downstream signaling molecules significantly differed after the T. annulata infection in comparison with that of preinfection from 72 h to 168 h postinoculation. The serum concentrations of IL-6, IL-1 beta, and TNF alpha were significantly increased at 96 h and 168 h postinfection. These findings provided novel information to help determine the mechanisms of TLR and NLR signaling pathway involvement in protection against T. annulata infection.

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