Clomazone influence soil microbial community and soil nitrogen cycling

文献类型： 外文期刊

第一作者： Wu, Xiaohu

作者： Wu, Xiaohu;Xu, Jun;Dong, Fengshou;Liu, Xingang;Zheng, Yongquan;Du, Pengqiang;Wu, Xiaohu;Xu, Jun;Zhang, Ying

作者机构：

关键词： Clomazone; Microbial community structure; nifH; amoA; Nitrogen cycling

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：7.963； 五年影响因子：7.842 ）

ISSN： 0048-9697

年卷期： 2018 年 644 卷

页码：

收录情况： SCI

摘要： We designed an indoor mesocosm experiment to investigate the long-term effects of exposure to clomazone, a widely used herbicide, on soil microbial communities and their nitrogen (N) cycling functions. Clomazone was applied to two typical soils from China at three concentrations: 0.8 (the recommended dosage), 8 and 80 mg kg(-1) soil dry weight, and the mix was incubated for 90 days. Samples were removed periodically for assay with several techniques. The half-lives of clomazone in this experiment were 11-126 d. Results were significant only for the highest clomazone concentration. Next-generation sequencing of the 16S and 18S rDNA genes revealed that bacterial diversity significantly decreased whereas fungal abundance increased after day 60 but with no detectable effect on the microbial community. Hierarchical cluster and principal coordinates analysis revealed that the bacterial community structure was negatively impacted. Linear discriminant analysis of effect size identified Sphingomonas and Arthrobacter as the predominant bacterial species. Finally, we measured soil NH4+ and NO3- concentrations and used real-time PCR to analyze the abundance of the N-cycling genes, nifH and amoA. In the first 30 days, the NO3--N content and the number of ammonia-oxidizing bacteria increased. N-2-fixing bacteria were inhibited after 60 days, but the NH4+-N concentration remained unchanged and was likely provided by ammoniation. (c) 2018 Elsevier B.V. All rights reserved.

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