A Simple, Sensitive, and Reliable Method for the Simultaneous Determination of Multiple Antibiotics in Vegetables through SPE-HPLC-MS/MS

文献类型： 外文期刊

第一作者： Feng, Yao

作者： Feng, Yao;Zhang, Wen-Juan;Liu, Yuan-Wang;Zhang, Shu-Qing;Li, Zhao-Jun;Feng, Yao;Liu, Yuan-Wang;Li, Zhao-Jun;Feng, Yao;Xue, Jian-Ming

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关键词： antibiotics; vegetable; SPE; HPLC-MS/MS

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2018 年 23 卷 8 期

页码：

收录情况： SCI

摘要： Antibiotics, widely used in livestock breeding, enter the environment through animal manure because of incomplete absorption in animals, especially the farmland ecosystem. Therefore, antibiotics may be adsorbed by plants and even become hazardous to human health through the food chain. In this study, a simple, sensitive, and reliable method was developed for the simultaneous determination of eleven antibiotics, including four sulfonamides, two tetracyclines, three fluoroquinolones, tylosin, and chloramphenicol in different vegetable samples using SPE-HPLC-MS/MS. Vegetable samples were extracted by acetonitrile added with hydrochloric acid (125:4, v/v). The extracts were enriched by circumrotating evaporation, and then cleaned through SPE on a hydrophilic-lipophilic balance (HLB) cartridge. All compounds were determined on a C-18 reverse phase column through HPLC-MS/MS. The mean recoveries of 11 antibiotics from spiked samples of vegetables ranged from 71.4% to 104.0%. The limits of detection and quantification were 0.06-1.88 mu g/kg and 0.20-6.25 mu g/kg, respectively. The applicability of this technique demonstrated its good selectivity, high efficiency, and convenience by the analysis of 35 vegetable samples available from a vegetable greenhouse. Antibiotic residues in vegetables have aroused wide concern from the public. Therefore, standards should be established for antibiotic residues in vegetables to ensure food safety and human health.

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