MiR-378 and BMP-Smad can influence the proliferation of sheep myoblast

文献类型： 外文期刊

第一作者： Lu, Zengkui

作者： Lu, Zengkui;Du, Lixin;Liu, Ruizao;Di, Ran;Li, Qing;Liu, Enmin;Chu, Mingxing;Wei, Caihong;Lu, Zengkui;Zhang, Liping;Ma, Youji

作者机构：

关键词： miR-378; BMP-Smad; Sheep myoblast; Myogenic regulation

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 674 卷

页码：

收录情况： SCI

摘要： MicroRNA (miRNA) is a sort of endogenous similar to 20-25 nt non-coding RNAs, and it can regulate a variety of biological events. We found the miR-378 may involve in regulating the muscle development of sheep during our previous research. However, the molecular mechanism of miR-378 regulating myoblast proliferation is still unclear. In this research, we predicted that BMP2 (Bone morphogenetic protein 2) was the target gene of miR-378 and the BMP-Smad signal pathway that BMP2 participated in playing an important role in the muscle development. Therefore, we tried to determine whether miR-378 influence myoblast proliferation of sheep through the BMP-Smad signal pathway. The results indicated that inhibit BMP-Smad signal pathway by interfering Smad4 to promote proliferation of sheep myoblasts; promote BMP-Smad signal pathway by interfering Smad7 to inhibit proliferation of sheep myoblasts; over-expression miR-378 promotes BMP-Smad signal pathway and myoblast proliferation in sheep; interfering miR-378 inhibits BMP-Smad signal pathway and myoblast proliferation in sheep. However, when both of which functioned at the myoblast, miR-378 could not fully depend on BMP-Smad signal pathway to regulate myoblast proliferation. In sum, both miR-378 and BMP-Smad can influence the proliferation of myoblast, but miR-378 does not target the 3' UTR of sheep BMP2.

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