文献类型： 外文期刊

第一作者： Xu Dong-po

作者： Xu Dong-po;Hu Hao-yuan;Xu Dong-po;Fang Di-an;Zhao Chang-sheng;Jiang Shu-lun

作者机构：

关键词： Takifugu obscures; HSP90 beta 1; TBT-C1 exposure; Immunologic function

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 666 卷

页码：

收录情况： SCI

摘要： HSP90 beta 1 (known as glyco-protein 96, GP96) is a vital endoplasmic reticulum (ER) depended chaperonin among the HSPs (heat shock proteins) family. Furthermore, it always processes and presents antigen of the tumor and keeps balance for the intracellular environment. In the present study, we explored the effect of tributyltin chloride (TBT-Cl) exposure on HSP90 beta 1 expression in river pufferfish, Takifugu obscurus. The full length of To-HSP90 beta 1 was gained with 2775 bp in length, with an ORF (open reading frame) encoding an 803 aa polypeptide. A phylogenetic tree was constructed and showed the close relationship to other fish species. The HSP90 beta 1 mRNA transcript was expressed in all tissues investigated with higher level in the gill and liver. After the acute and chronic exposure of TBT-Cl, the To-HSP90 beta 1 mRNA transcript significantly was up-regulated in gills. Moreover, the histology study indicated the different injury degree of TBT-Cl in liver and gill. Immunohistochemistry (IHC) staining results implied the cytoplasm reorganization after TBT-Cl stress and the function of immunoregulation for To-HSP90 beta 1 to TBT-Cl exposure. All the results indicated that HSP90 beta 1 may be involved in the resistance to the invasion of TBT-CI for keeping autoimmune homeostasis.

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