Methyl jasmonate regulates the glycolytic pathway and the PpbZIP43-PpFAD2 module to enhance cold resistance in peach fruit

文献类型： 外文期刊

第一作者： Wang, Lufan

作者： Wang, Lufan;Wang, Yuxuan;Mao, Jiaxu;Dai, Binger;Zhou, Liang;Zhang, Shiying;Shen, Shuling;Zheng, Xiaolin;Huan, Chen;Zhou, Huijuan;Wang, Libin

作者机构：

关键词： BZIP transcription factor; Chilling injury; Fatty acid desaturation; Glycolysis; Methyl jasmonate; Peach

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.8； 五年影响因子：7.5 ）

ISSN： 0925-5214

年卷期： 2025 年 228 卷

页码：

收录情况： SCI

摘要： Peach (Prunus persica L.) fruit is highly susceptible to chilling injury (CI) during cold storage, severely affecting its commercial value. This study investigated the molecular mechanism of methyl jasmonate (MeJA) in alleviating CI symptoms in 'Hujingmilu' peach fruit. Transcriptome analysis revealed that MeJA treatment enhanced glycolytic metabolic activity by upregulating key glycolytic genes (PpGAPDH, PpPK, PpGPML, PpALDO, and PpENO), potentially contributing to the increased NADH content and elevated NADH/NAD+ ratio. Concurrently, MeJA activated the NADH-cytochrome b5 reductase (CBR)-cytochrome b5 (CB5)-fatty acid desaturase (FAD) fatty acid desaturation chain by upregulating PpCBR and PpFAD2 expression. These results suggest that MeJA may provide sufficient reducing power for the fatty acid desaturation process during cold storage of peach fruit. Additionally, MeJA induced the expression of transcription factor PpbZIP43. Yeast one-hybrid and electrophoretic mobility shift assays demonstrated that PpbZIP43 directly binds to the G-box motif within the PpFAD2 promoter region. Dual-luciferase reporter assays confirmed that PpbZIP43 functions as a transcriptional activator of PpFAD2. Transient overexpression and silencing of PpbZIP43 further verified that PpbZIP43 positively regulated PpFAD2 expression in peach fruit. In summary, MeJA potentially enhances cold tolerance in peach fruit through dual mechanisms: enhancing NADH-dependent fatty acid desaturation chain and promoting PpbZIP43-mediated transcriptional activation. These pathways collectively facilitate fatty acid desaturation, resulting in increased unsaturated fatty acid content and an improved unsaturated/saturated fatty acid ratio, thereby enhancing cell membrane stability under cold stress conditions.

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