A single amino acid polymorphism in ABCC2 loop 1 is responsible for differential toxicity of Bacillus thuringiensis Cry1Ac toxin in different Check for Spodoptera (Noctuidae) species

文献类型： 外文期刊

第一作者： Liu, Leilei

作者： Liu, Leilei;Chen, Zuwen;Yang, Yanchao;Ma, Yuemin;Yang, Yongbo;Liu, Kaiyu;Xiao, Yutao;Liu, Chenxi;Soberon, Mario;Bravo, Alejandra

作者机构：

关键词： Spodoptera litura; Spodoptera frugiperda; Helicoverpa armigera; ABCC2 transporter; Bacillus thuringiensis; Cry toxin-resistance

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2018 年 100 卷

页码：

收录情况： SCI

摘要： Bacillus thuringiensis Cry toxins exert their toxicity by forming membrane pores after binding with larval midgut membrane proteins known as receptors. Spodoptera litura and Spodoptera frugiperda belong to the same genus, but S. litura is tolerant to Cry1Ac, while S. frugiperda is susceptible. The mechanism involved in the differential toxicity of Cry1Ac to these insect species is not understood. Amino acid sequences analysis of ABCC2, a well-recognized Cry1Ac receptor, from both species showed high sequence identity. Hi5 insect cells expressing SfABCC2 from S. frugiperda were 65-fold more susceptible than those expressing the SlABCC2 from S. litura. Substitution of fragments, point mutations and deletions between the ABCC2 of the two species revealed that ABCC2 amino acid Q(125) from SfABCC2 or E-125 from SlABCC2 was key factor for the differential Cry1Ac toxicity to Hi5 cells expressing these receptors. Consistently with this, cells expressing Helicoverpa armigera HaABCC2(Q122)-GFP, were more susceptible to Cry1Ac than cells expressing HaABCC2(E122)-GFP mutant. Q(125) or E-125 is located in a predicted exposed loop 1 region of ABCC2 indicating that this region could be important for Cry1Ac binding. These findings identified a single amino acid residue located in loop 1 of ABCC2 transporter as responsible for the different levels of susceptibility to Cry1Ac among various lepidopteran species.

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