Cloning and characterization of MdGST1 from red apple leaves

文献类型： 外文期刊

第一作者： Han, Xiaolei

作者： Han, Xiaolei;Zhang, Caixia;Tian, Yi;Gul, Hera;Cong, Peihua;Zhang, Liyi

作者机构：

关键词： apple; anthocyanin; glutathione S-transferase; transport

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2018 年 98 卷 5 期

页码：

收录情况： SCI

摘要： Glutathione S-transferase (GST) is involved in the downstream steps of the anthocyanin biosynthesis pathway in plants. However, the gene(s) encoding this enzyme have not been isolated from apple (Malus x domestica Borkh.) yet. We isolated a gene encoding GST from leaves of the red-fleshed apple variety 'Royalty' by full cDNA library sequencing and the 3' rapid-amplification of cDNA ends method, and designated it MdGST1. In total, seven different MdGST1 transcripts were found. These had three different untranslated but identical protein-coding regions. Phylogenetic analysis showed that MdGST1 is a TT19-type GST, which is involved in anthocyanin transport. qRT-PCR analyses showed that the transcript level of MdGST1 was much higher in red leaves than in bagged or green leaves. When MdGST1 was introduced into a non-pigmented mutant of Arabidopsis, the transformants showed a visible purple phenotype in leaves and stems. Our results suggest that MdGST1 plays a role in anthocyanin biosynthesis. This information will help to improve the understanding of the mechanism of apple coloration.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序