Gastrointestinal Metagenomics of Fish: Methods, Research Advances and Applications

文献类型： 外文期刊

第一作者： Dong, Bo

作者： Dong, Bo;Deng, Deng;Dong, Bo;Yi, Yunhai;Zhao, Xiaomeng;Shi, Qiong;Yi, Yunhai;Zhao, Xiaomeng;You, Xinxin;Shi, Qiong;Zhao, Xiaomeng;You, Xinxin;Shi, Qiong

作者机构：

关键词： Antimicrobial peptide; Fish; Gastrointestinal microbiota; Metagenomics; Next-generation sequencing; Probiotics

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2018 年 20 卷 10 期

页码：

收录情况： SCI

摘要： Fish microbiota function in fish nutrition and health, but research has been hindered by difficulties in culturing these microorganisms. New molecular techniques, like DDGE (Denaturation gradient gel electrophoresis) and FISH (Fluorescence in situ Hybridization), have produced limited detailed quantitative results. Next-generation sequencing based metagenomics and microarray offers more promise. These high throughput methods can obtain the overall genetic information within a microorganism community and there is no need to cultivate the individual microbes in advance. Metagenomics combines the sequencing of 16S rRNA genes and genomes of the sampled microbiota. We provide an overview of recent research advances in fish metagenomics and related applications. Many studies have already been done on individual fish species, but it is necessary to obtain a comprehensive profile of the microbiota of all fish species using metagenomics sequencing. (C) 2018 Friends Science Publishers

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