MOV10 inhibits replication of porcine reproductive and respiratory syndrome virus by retaining viral nucleocapsid protein in the cytoplasm of Marc-145 cells

文献类型： 外文期刊

第一作者： Zhao, Kuan

作者： Zhao, Kuan;Li, Li-Wei;Zhang, Yu-Jiao;Jiang, Yi-Feng;Gao, Fei;Li, Guo-Xin;Yu, Ling-Xue;Zhao, Wen-Ying;Shan, Tong-Ling;Zhou, Yan-Jun;Tong, Guang-Zhi;Li, Li-Wei;Jiang, Yi-Feng;Gao, Fei;Li, Guo-Xin;Yu, Ling-Xue;Shan, Tong-Ling;Zhou, Yan-Jun;Tong, Guang-Zhi

作者机构：

关键词： Porcine reproductive and respiratory; syndrome virus; Moloney leukemia virus 10-like protein; Nucleocapsid protein; Viral replication

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2018 年 504 卷 1 期

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) has been a major threat to global industrial pig farming ever since its emergence in the late 1980s. Identification of sustainable and effective control measures against PRRSV transmission is a pressing problem. The nucleocapsid (N) protein of PRRSV is specifically localized in the cytoplasm and nucleus of virus-infected cells which is important for PRRSV replication. In the current study, a new host restricted factor, Moloney leukemia virus 10-like protein (MOV10), was identified as an inhibitor of PRRSV replication. N protein levels and viral replication were significantly reduced in Marc-145 cells stably overexpressing MOV10 compared with those in wild-type Marc-145 cells. Adsorption experiments revealed that MOV10 did not affect the attachment and internalization of PRRSV. Co-immunoprecipitation and immunofluorescence co-localization analyses showed that MOV10 interacted and co-localized with the PRRSV N protein in the cytoplasm. Notably, MOV10 affected the distribution of N protein in the cytoplasm and nucleus, leading to the retention of N protein in the former. Taken together, these findings demonstrate for the first time that MOV10 inhibits PRRSV replication by restricting the nuclear import of N protein. These observations have great implications for the development of anti-PRRSV drugs and provide new insight into the role of N protein in PRRSV biology. (C) 2018 Elsevier Inc. All rights reserved.

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