Gga-miR-205a Affecting Myoblast Proliferation and Differentiation by Targeting CDH11

文献类型： 外文期刊

第一作者： Wang, Zhijun

作者： Wang, Zhijun;Ouyang, Hongfia;Chen, Xiaolan;Yu, Jiao;Abdallat, Bahareldin A.;Chen, Biao;Nie, Qinghua;Wang, Zhijun;Chen, Xiaolan;Yu, Jiao;Abdallat, Bahareldin A.;Chen, Biao;Nie, Qinghua;Wang, Zhijun;Chen, Xiaolan;Yu, Jiao;Abdallat, Bahareldin A.;Chen, Biao;Nie, Qinghua;Wang, Zhijun;Chen, Xiaolan;Yu, Jiao;Abdallat, Bahareldin A.;Chen, Biao;Nie, Qinghua;Ouyang, Hongfia

作者机构：

关键词： miR-205a; CDH11; myoblast; myogenesis; promoter active region

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Non-coding RNAs especially miRNAs have been found to play important roles during skeletal muscle development. Our previous RNA-Seq performed on breast muscle tissue from 7 weeks old Recessive White Rock and Xinhua Chicken and leg muscle tissue from female Xinghua Chicken at three development time points (11 embryo age, 16 embryo age, and 1 day post hatch) (accession number GSE62971 and GSE89355, respectively) showed that miR-205a and CDH11 were differentially expressed genes. In this study, we found that overexpression of CDH11 significantly facilitated Quail muscle clone (QM7) and chicken primary myoblast (CPM) proliferation and hampered CPM differentiation. MiR-205a can directly binding to the 3'UTR of CDH11 and the overexpression of miR-205a could inhibit both cell lines (QM7) and CPM proliferation, at the meantime promote the differentiation of myoblasts. The Dual-Luciferase Reporter Assay results and qRT-PCR results showed that myogenin (MyoG) could regulate the expression of miR-205a by binding to the active region of miR-205a. Altogether our data suggest that MyoG could stimulate miR-205a expression to suppress CDH11, which promotes myoblasts proliferation while represses the differentiation.

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