The role of the veA gene in adjusting developmental balance and environmental stress response in Aspergillus cristatus

文献类型： 外文期刊

第一作者： Tan, Yumei

作者： Tan, Yumei;Wang, Yaping;Ren, Chunguang;Tan, Yumei;Wang, Hai;Wang, Yaping;Ren, Chunguang;Wang, Yuchen;Ren, Xiyi;Liu, Yongxiang;Liu, Zuoyi;Tan, Yumei;Wang, Yuchen;Ren, Xiyi;Liu, Yongxiang;Ge, Yongyi;Ren, Xiuxiu;Liu, Zuoyi

作者机构：

关键词： Asexual development; RNA-Seq; Sexual sporulation; velvet gene

期刊名称：FUNGAL BIOLOGY （ 影响因子：3.099； 五年影响因子：3.364 ）

ISSN： 1878-6146

年卷期： 2018 年 122 卷 10 期

页码：

收录情况： SCI

摘要： veA belongs to the velvet regulatory system that regulates the development and secondary metabolism of many fungi. To identify the function of veA in Aspergillus cristatus, veA deletion mutants were constructed by homologous recombination via Agrobacterium tumefaciens-mediated transformation. Deletion of veA led to increased conidial production and reduced sexual sporulation. The regulatory role of veA in A. cristatus was not light-dependent, and this differed from its role in other Aspergilli. Furthermore, veA deletion mutants were more sensitive to environmental stressors, including salt, osmotic pressure, temperature and pH. In contrast, deletion of veA resulted in increased resistance to oxidative stress. veA also affected aerial vegetative growth. Transcriptomic analysis of the veA-null mutant and wild type indicated that most asexual and sexual development genes were upregulated and downregulated, respectively. These findings confirmed that veA has a positive effect on sexual development but represses conidial formation. Overall, these results suggested that the veA gene plays a critical role in maintaining a developmental balance between asexual and sexual sporulation and is involved in vegetative growth and environmental stress response in A. cristatus. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

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