Mutation-388 C > G of NR5A1 gene affects litter size and promoter activity in sheep

文献类型： 外文期刊

第一作者： Li, Yinxia

作者： Li, Yinxia;Zhang, Jun;Qian, Yong;Meng, Chunhua;Wang, Huili;Cao, Shaoxian;Li, Yinxia;Zhang, Jun;Qian, Yong;Meng, Chunhua;Wang, Huili;Cao, Shaoxian;Li, Yinxia;Zhang, Jun;Qian, Yong;Meng, Chunhua;Wang, Huili;Cao, Shaoxian

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关键词： Hu sheep; NRSA1; Core promoter; SNP; CpG site; SP1

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN： 0378-4320

年卷期： 2018 年 196 卷

页码：

收录情况： SCI

摘要： The Nuclear receptor superfamily 5, group A, member 1 (NR5A1) gene encodes a nuclear receptor that regulates the transcription of genes involved in steroidogenesis, follicular development and female fertility. Little, however, is known about the relationship of this gene with reproductive performance in sheep. In this study, the transcription initiation site of Hu sheep NR5A1 gene was located 193 nucleotides (i.e., at -193 nt) before the translational start site (ATG). The core promoter region of the NR5A1 gene ranged from -696 nt to - 298 nt, and a C > G mutation at -388 nt was detected in this region. Association analysis indicated ewes with the GG genotype had greater litter size at the second and third parity than those with the CC genotype (P < 0.05). The results from the luciferase assay provided evidence that the -388 G allele increased luciferase activity compared with that of the - 388 C allele. Furthermore, the -388 C > G mutation lost a CpG site and gained a novel binding site for the transcription factor, SP1, and results from an overexpression experiment and methylation analysis indicated transcription factor SP1 and methylation of the -388 C > G mutation were both involved in alteration of NR5A1 transcription activity. Results of the present study revealed that the -388 C > G mutation lost a CpG site and promoted NR5A1 gene expression, which completely superimposed positive effects on NR5A1 gene transcription activity by transcription factor SP1, resulting in a fecundity increase in Hu sheep.

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