Glycyrrhizic acid activates chicken macrophages and enhances their Salmonella-killing capacity in vitro

文献类型： 外文期刊

第一作者： Wang, Bai-kui

作者： Wang, Bai-kui;Mao, Yu-long;Gong, Li;Xu, Xin;Wang, Yi-bing;Li, Wei-fen;Jiang, Shou-qun

作者机构：

关键词： Glycyrrhizic acid; Chicken macrophage; Macrophage activation; Salmonella Typhimurium; Nuclear factor B (NF-B); c-Jun N-terminal kinase (JNK)

期刊名称：JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B （ 影响因子：3.066； 五年影响因子：3.057 ）

ISSN： 1673-1581

年卷期： 2018 年 19 卷 10 期

页码：

收录情况： SCI

摘要： ObjectiveSalmonella enterica remains a major cause of food-borne disease in humans, and Salmonella Typhimurium (ST) contamination of poultry products is a worldwide problem. Since macrophages play an essential role in controlling Salmonella infection, the aim of this study was to evaluate the effect of glycyrrhizic acid (GA) on immune function of chicken HD11 macrophages.MethodsChicken HD11 macrophages were treated with GA (0, 12.5, 25, 50, 100, 200, 400, or 800 g/ml) and lipopolysaccharide (LPS, 500 ng/ml) for 3, 6, 12, 24, or 48 h. Evaluated responses included phagocytosis, bacteria-killing, gene expression of cell surface molecules (cluster of differentiation 40 (CD40), CD80, CD83, and CD197) and antimicrobial effectors (inducible nitric oxide synthase (iNOS), NADPH oxidase-1 (NOX-1), interferon- (IFN-), LPS-induced tumor necrosis factor (TNF)- factor (LITAF), interleukin-6 (IL-6), and IL-10), and production of nitric oxide (NO) and hydrogen peroxide (H2O2).ResultsGA increased the internalization of both fluorescein isothiocyanate (FITC)-dextran and ST by HD11 cells and markedly decreased the intracellular survival of ST. We found that the messenger RNA (mRNA) expression of cell surface molecules (CD40, CD80, CD83, and CD197) and cytokines (IFN-, IL-6, and IL-10) of HD11 cells was up-regulated following GA exposure. The expression of iNOS and NOX-1 was induced by GA and thereby the productions of NO and H2O2 in HD11 cells were enhanced. Notably, it was verified that nuclear factor-B (NF-B) and c-Jun N-terminal kinase (JNK) pathways were responsible for GA-induced synthesis of NO and IFN- gene expression.ConclusionsTaken together, these results suggested that GA exhibits a potent immune regulatory effect to activate chicken macrophages and enhances Salmonella-killing capacity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序