Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional Food

文献类型： 外文期刊

第一作者： Ren, Guixing

作者： Ren, Guixing;Zhao, Gang;Ren, Guixing;Hao, Yuqiong;Zhu, Yingying;Shi, Zhenxing;Zhu, Yingying;Shi, Zhenxing;Zhao, Gang

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关键词： lunasin; transgenic rice; UPLC-MS/MS; antioxidant activity; anti-inflammatory activity

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2018 年 23 卷 9 期

页码：

收录情况： SCI

摘要： Lunasin, a bioactive peptide initially isolated from soybean, has anticancer, anti-inflammatory, and antioxidant activity. Due its great application value, lunasin seems to be a candidate gene in improving the nutritional value of crops. In this study, lunasin was inserted into the rice genome to evaluate whether it was feasible to express lunasin using the rice expression system and improve the bioactivity of protein in rice for our needs. We generatedlunasin-overexpressing rice lines, and chose three independent transgenic rice lines for further study. The lunasin content in trans-lunasin rice detected by UPLC-MS/MS was 1.01 x 10(-3) g.kg(-1) dry rice flour with grease removal in the lunasin extracts. The antioxidant efficacy of LET (lunasin-enriched fraction from trans-lunasin rice) and PEW (peptide-enriched fraction from wild type rice) was compared. Due to the presence of lunasin, LET showed higher (p < 0.05) antioxidant activity than PEW. LET exhibited high DPPH radical scavenging activity (IC50 value, 8 g.L-1), strong ABTS(+) radical scavenging activity (IC50 value, 1.18 g.L-1), and great oxygen radical scavenging activity (170 mu mol.L-1 Trolox equivalents when the concentration reached 4 g.L-1). Moreover, LET presented significantly higher (p < 0.05) anti-inflammatory activity on macrophage cells, and the NO production and the release of pro-inflammatory cytokines (IL-6, MCP1, and TNF-alpha) feasible to use the rice expression system to express the exogenous lunasin in rice, and lunasin-overexpressing rice seems to be a candidate resource for application in functional food. Rice rich in lunasin is beneficial for human health, and could be used as a functional food in the diets of cancer and obese patients in the future.

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