Different glyceollin synthesis-related metabolic content and gene expressions in soybean callus suspension cultures and cotyledon tissues induced by alginate oligosaccharides

文献类型： 外文期刊

第一作者： Wang, Kaiqiang

作者： Wang, Kaiqiang;Peng, Qing;Qiao, Yu;Shi, Bo;Li, Yang;Suo, Decheng

作者机构：

关键词： Glyceollin; Elicitation; Callus suspension cultures; Cotyledon tissues; Alginate oligosaccharides; Glycine max

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2018 年 73 卷

页码：

收录情况： SCI

摘要： Glyceollins are a group of inducible soybean phytoalexins. The aim of the present study was to investigate the mechanism of glyceollin production in callus suspension cultures (CS) and cotyledon tissues elicited with alginate oligosaccharides (AOS) at 1%, 2%, and 3% concentrations that were applied for three days. HPLC analysis of the extracts from 2% AOS-elicited callus suspension cultures (ECS) revealed that glyceollins content was 85.3-fold and 8.2-fold higher than that in unelicited controls and elicited cotyledon tissues (ECT), respectively. UPLC-Q-Exactive Orbitrap MS analysis showed that the 2% AOS induction resulted in elevated levels of metabolites, the intermediary products of glyceollin biosynthesis, such as daidzein, glycitein, genistein, naringenin, glycinol, glyceollidin I/II, and glyceollin I, II, and III in ECS and ECT. Glyceollin I was prominent among the three glyceollin isomers in ECS, whereas isomer II and III were dominant in the synthetic route for glyceollins in ECT. qRT-PCR analysis of related genes showed that UGT, CYP93A1, G2DT, and G4DT displayed a direct effect on glyceollin synthesis. Our results suggested that 2% AOS-induced CS produced glyceollins in higher content compared to ECT by altering metabolite content and gene expression. Elicitation of CS is thus a promising technology for glyceollin synthesis extensively.

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