Systematic investigation of circular RNAs in Ascosphaera apis, a fungal pathogen of honeybee larvae

文献类型： 外文期刊

第一作者： Guo, Rui

作者： Guo, Rui;Chen, Dafu;Chen, Huazhi;Fu, Zhongmin;Xiong, Cuiling;Zheng, Yanzhen;Guo, Yilong;Wang, Haipeng;Du, Yu;Hou, Chunsheng;Diao, Qingyun

作者机构：

关键词： circRNA; Ascosphaera apis; Fungal pathogen; Honeybee larvae

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 678 卷

页码：

收录情况： SCI

摘要： Ascosphaera apis is a widespread fungal pathogen of honeybee larvae, which causes heavy losses in apiculture. To date, knowledge about non-coding RNA (ncRNA) including circular RNA (circRNA) in A. apis is lacking. In this study, A. apis mycelia and spores were sequenced using RNA-seq technology. A total of 551 circRNAs were predicted on the basis of bioinformatic analyses, and most of the circRNAs were 200-600 bp in length, which were different from animal and plant circRNAs. In addition, the expression of six circRNAs in A. apis were confirmed using divergent and convergent PCR. Moreover, circRNA-microRNA regulation networks in A. apis were constructed, and further investigation showed that A. apis circRNAs could regulate gene expression by competitively binding miRNAs. GO and KEGG pathway enrichment analyses of the miRNAs target genes of circRNAs demonstrated that these A. apis circRNAs are likely to play key roles in metabolism, environmental response and gene expression.

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