Isolation and Characterization of 19 Polymorphic Microsatellite Markers of Sepia esculenta

文献类型: 外文期刊

第一作者: Meng, Xiangbin

作者: Meng, Xiangbin;Song, Na;Liu, Lu;Zhang, Xiumei;Gao, Tianxiang;Liu, Liqin;Li, Chunhou;Li, Chunhou

作者机构:

关键词: Sepia esculenta; Population structure; Genetic diversity; Polymorphic loci; Microsatellite makers

期刊名称:PAKISTAN JOURNAL OF ZOOLOGY ( 影响因子:0.831; 五年影响因子:0.768 )

ISSN: 0030-9923

年卷期: 2018 年 50 卷 4 期

页码:

收录情况: SCI

摘要: Sepia esculenta is an economically important marine cephalopoda. The availability of highly polymorphic markers will be important to promote the conservation of this species. We performed next-generation sequencing and de novo assembly to obtain potential useful microsatellite markers for S. esculenta. Among 80 tested microsatellite markers, 19 showed polymorphism among S. esculenta individuals. The allele number of all polymorphic microsatellite markers ranged from 6 to 10. Expected and observed heterozygosity varied from 0.260 to 0.904 and from 0.292 to 0.861, with an average of 0.741 and 0.607, respectively. The polymorphism information content (PIC) ranged from 0.566 to 0.823. These markers will be useful in further studies and will give new insight into conservation and efficient management of this species.

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